Current Issue : January-March Volume : 2011 Issue Number : 1 Articles : 9 Articles
Acorus calamus is a valued drug used extensively in Ayurvedic therapeutics. Due to high market demand, Acorus is being cultivated. Hence the study was undertaken to analyze the morphological and chemical characteristics of Acorus calamus Linn. especially β-asarone collected from its cultivated as well as wild sites and demonstrate any similarities seen. The results of the study indicated that there is no much difference in the morphological features and phyto chemical parameters. GC-MS revealed β-asarone was moderately high in wild variety (85.34%) compared to cultivated one (78.49 %) which could be attributed to environmental factors. Pharmacological study is desirable for greater comparability of the results obtained to assure the usage of cultivated variety....
A simple, selective, precise and stability-indicating high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for analysis of Montelukast sodium (MTKT) and Bambuterol hydrochloride(BAM) both as the bulk drugs and in formulations. The compounds were separated on aluminum-backed silica gel 60 F254 plates with toluene: ethanol: diethyl amine (9: 0.4: 0.6 v/v/v) as mobile phase. This system was found to give compact spots for MTKT and BAM (Rf values 0.43 ± 0.02 and 0.65 ± 0.04, respectively). Densitometric analysis of MTKT and BAM was performed at 266 nm. Regression analysis data for the calibration plots were indicative of good linear relationships between response and concentration over the range of 50–600 ng per spot for MTKT and 100-1200 ng per spot for BAM. The correlation coefficients, r2, were 0.9970 and 0.9950 for MTKT and BAM, respectively. The values of slope and intercept of the calibration plots were 5.942 ± 0.22 and 743.42 ± 51.38 for MTKT and 2.137 ± 0.25 and 381.26 ± 61.11 for BAM, respectively. The method was validated for precision, recovery and robustness. The limits of detection and quantification were 18.0 ± 0.51 and 50 ± 1.05 ng per spot for MTKT and 30 ± 0.63 and 100 ± 1.12 ng per spot for BAM, respectively. MTKT and BAM were subjected to acid, base, peroxide and photo degradation. In stability tests the drugs were susceptible to acid and basic hydrolysis, oxidation and photolytic degradation. Statistical analysis proved the method is repeatable, selective and accurate for estimation of MTKT and BAM because the method could effectively separate the drugs from their degradation products, so it can be used as a stability-indicating method....
Two simple and sensitive visible spectrophotometric methods (A-B) for the determination of Cefprozil (CEF) in bulk sample and pharmaceutical formulations are described. Methods A and B are based on the formation of ion-association complex involving carboxylic acid group of CEF and the basic dyes, safranin-O (SFN-O, method A), methylene blue (MB, method B). The results obtained in the two methods are reproducible and are statistically validated and found to be suitable for the assay of Cefprozil in bulk and pharmaceutical formulations....
A simple, sensitive, accurate, rapid, and economical spectrophotometric method has been developed for the determination of PPX in bulk drug and pharmaceutical formulations. The method is based on the reduction of iron (III) by the drug and subsequent interaction of iron (II) with ferricyanide to form green colored chromogen. The product exhibit maximum absorbance at 789.0 nm against reagent blank. Beer's law was obeyed in the concentration range of 1.0-5.0 µg/ml with correlation coefficient (r2) of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.082 µg/ml and 0.249 µg/ml, respectively. Results of the analysis were validated statistically and by recovery studies. The results are compared with previously developed UV spectrophotometric method. Commercial tablet formulation was successfully analyzed using the developed method....
There are number of plants and classical Polyherbal formulations reported in market but standardization to support their efficacy are lacking. Large numbers of therapies are based on the herbs and their formulations. At present Validation of such plants has become too much essential to represent them in the modern context with their efficacy. In the present investigation, one Polyherbal formulation (Neorise granules) consists of aqueous extract of Brahmi (Centella asiatica) and Shankhapushpi (Evolvulus alsinoides). Here estimation is done by isolating Asiaticoside, which is quantified by HPTLC method. All the above mentioned plants were identified by its morphological and microscopical analysis and physicochemical evaluation. Preliminary phytochemical screening revealed the presence of alkaloid, tannin, phenolic compounds, Saponin, Flavanoids, Glycosides, Triterpenoid and bitter principles. TLC densitometric methods were developed using HPTLC for the quantification of Asiaticoside present in polyherbal formulation. Asiaticoside content was 0.0054 ± 0.01% in Polyherbal formulation (Neorise granules). The method was validated in terms of Linearity, Precision, and Limit of detection, Limit of quantification, Accuracy and specificity....
Multi-Elemental Analysis of two Anti-diabetics Plant Species: Hemidesmus indicus (L.) R.Br. and Hybanthus enneaspermus (L.) F.V. Muell and their Callus Cultures using EDXRF Technique...
This review highlights liquid chromatography in conjunction with mass spectroscopy (LC-MS) also the brief overview on development of its interfaces, mass analyzers are discussed. The presented report touches applications of LC-MS to pharmaceutical quantitative analysis in various areas like drug discovery, metabolism studies, analysis and identification of impurities and degradation products in pharmaceuticals. Presented report also details working and instrumentation related aspects of hyphenated technology, liquid chromatography in conjunction with nuclear magnetic resonance spectroscopy (LC-NMR). The report addresses the experimental setup and major contribution of LC-NMR to various areas in pharmaceutical analysis in fields like drug metabolism and their contribution to studies to natural products is also discussed....
Two simple, sensitive, accurate and economical spectrophotometric methods were developed for the estimation of mebevrine hydrochloride (MB) and chlordiazepoxide (CLR) simultaneously in tablet dosage form.First method is based on the simultaneous equations and second method is based on Q-analysis (absorbance ratio method). Mebeverine hydrochloride and chlordiazepoxide shows absorbance maxima at 220.0 nm and 264.5 nm in methanol, respectively. The linearity was obtained in the concentration range of 2-12 µg/ml for MB and 1-10 µg/ml for CLR. In the first method, the concentrations of the drugs were determined by using simultaneous equations; and in the second method, the concentrations of the drugs were determined by using ratio of absorbance at isoabsorptive point and at the λmax of the one drug.Statistical validation of the data has been carried out and it reveals that the proposed method is sensitive and economical too for the routine analysis of the drugs in combined dosage form....
The influence of three different solvents on estimation of sumatriptan from bulk and pharmaceutical dosage forms was studied. The solutions of sumatriptan were prepared with the water and the solvent blends containing water: acetonitrile (1:1) and water: methanol (1:1). The solutions were scanned with in the ranges of 200 nm to 400nm. Sumatriptan exhibited max at 283 nm in case of water, however the max was slightly shifted to 283.5 nm with the solvents acetonitrile: water and methanol: water. The method obeyed Beer-Lamberts law with in the range of 10 to 50 g/ml concentration. The analytical parameters such as linearity, A (1%, 1 cm), LOD, LOQ, inter day precision, intraday precision, molar absorption coefficient, and sandells sensitivity were calculated. The results of analysis were validated by recovery studies. The recovery was more than 98%. The method was found to be simple, accurate, precise, economical and robust. The validation parameters were treated statistically with ANOVA and there were significant differences noticed. This study clearly indicated that the solvent is having any influence in determination of sumatriptan from bulk drug as well as from the formulations. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of sumatriptan succinate from pharmaceutical preparation....
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